Abstract
The potential of Near Infrared Spectroscopy (NIRS) in quality control of Traditional Chinese Medicine has been evaluated. This study focuses on a rapid and non-destructive quantitative method for the determination of schaftoside acid content in Desmodium styracifolium by near-infrared spectroscopy. The schaftoside acid contents of 103 samples were determined by HPLC. The NIRS quantitative model of the ethanol extracts content was performed by partial least-squares (PLS) regression as linear regression with analysis values as calibration reference using 2nd derivative method as spectral preprocessing options. The model was verified by internal cross validation and external predictive validation with 22 Desmodium styracifolium samples. The correlation coefficients (r), the root-mean-square error of calibration (RMSEC), the root-mean-square error of prediction (RMSEP) and the root-mean-square error of cross-validation (RMSECV) of the calibration model for ethanol extracts content were 0.97201, 0.147, 0.162 and 0.433, respectively. The average recovery was 99.82%, and t-test showed that P > 0.5. Precision and repeatability RSD were 1.75% and 1.17%, RPD > 2.5. The data obtained from results showed that calibration model of NIRS was verified to be reliable, suggesting that the quantitative NIR models can be qualified to accurately quantify the ethanol extracts content of Desmodium styracifolium rapidly.
Highlights
Introduction(D. styracifolium), a plant belonging to genus Desmodium, family Fabaceae, is a traditional Chinese medicine that
Phytochemical studies have shown that D. styracifolium contains alkaloids, flavonoids, glycosides, phenols, tannins and many other chemical components [6], of which schaftoside acid is an active component that recorded in the Chinese Pharmacopoeia of 2015 edition (ChP) for quality control of ingredients [7]
Multiplicative scatter correction (MSC) and standard normal variate (SNV) methods were used to eliminate the influence of radiation scattering customary, and 1st and 2nd derivative spectra were compared to solve the effects of baseline drift
Summary
(D. styracifolium), a plant belonging to genus Desmodium, family Fabaceae, is a traditional Chinese medicine that. It has been highlighted the use of its dry aerial parts as a cool-natured, sweet medicine for the treatment of many aliments like heat clearing, dampness, detumescence and kidney stone in clinical [1]. Traditional Chinese medicine showed the presence of different chemical components. They are hardly to be rapidly determined. Near Infrared Spectroscopy (NIR) technology has been widely applied in discrimination of Chinese medicinal material and Chinese medicinal preparations for its rapidity, non-destructiveness and accuracy [8] [9] [10]
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