Abstract
A novel and fast competitive direct biomimetic ELISA (cd-BELISA) was developed for determination of the N-methylcarbamate insecticide metolcarb based on a molecularly imprinted polymer (MIP) film as the antibody mimic. The MIP film was directly synthesized on the surface of a 96-well plate by bulk polymerization. The synthesized film was characterized, and the results showed that the imprinted film exhibited antibody-antigen-like binding properties and rapid adsorption ability, which was particularly useful for cd-BELISA development. The cd-BELISA conditions were optimized in detail. Under the optimum conditions, the sensitivity and LOD of the cd-BELISA were found to be 17 and 0.12 microg/L, respectively. Crossreactivity demonstrated that the cd-BELISA had a higher selectivity for metolcarb than for structurally related compounds. The developed method was applied to the determination of metolcarb in spiked apple juice, cabbage, and cucumber, with mean recoveries ranging from 71.5 to 117.0%. Validation of the results was conducted by HPLC with good correlation (r2 > 0.9562) between data obtained using these two methods. Therefore, the developed cd-BELISA has potential for the rapid determination of metolcarb in foods.
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
