Abstract
A rapid, sensitive, specific, and cost-effective method for the determination of N-nitrosodiethylamine (NDEA) in rodent tissue and body fluid samples is reported. The method involves isolation of the NDEA from the samples with subsequent detection and quantitation of observed NDEA against mass-labeled N-(15N)-nitrosodiethyl-(15N)-amine (15N2DEA) by high resolution gas chromatography/high resolution mass spectrometry selected ion monitoring. Validation of the method included linearity studies of NDEA response, calculation of the relative response factor (RRF) for NDEA versus 15N2DEA, and absolute recovery studies. Estimates of precision, detection limit, and minimum quantifiable limit are also reported.
Published Version
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