Abstract
A rapid method is presented for the quantitative analysis of lactose, glucose, and galactose in whey and whey permeate by high performance liquid chromatography. Samples were prepared by heating to precipitate most proteins and by eluting through a Sep-Pak™ cartridge to remove lipids, chromophores, and residual proteins. Sugars were separated on an Altex μ-Spherogel Carbohydrate column and measured by a refractive index detector. Recovery of added carbohydrates averaged 98.0% for lactose and 101.5% for glucose and galactose. Coefficients of variation for recovery ranged from .27 to 3.20%. Reproducibility was demonstrated by our monitoring rate of enzymatic hydrolysis of lactose in whey permeate. Sample preparation time was 15min; chromatographic separation was within 15min.
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Disclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.