Abstract
A rapid method for analysis of adenine nucleotides (AMP, ADP and ATP) in nervous tissue based on ion-paired reversed-phase column liquid chromatography under isocratic conditions is described. An optimal composition of elution buffer was 25 m M potassium phosphate and 4% triethylamine adjusted to pH 6:5 with phosphoric acid. Typical separation time did not exceed 10 min with a 10-cm long compact glass cartridge packed with 5μm silica C 18. The method was employed to determine ATP, ADP and AMP concentrations in rat brain extracts and values thus obtained were compared with those published elsewhere.
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callMore From: Journal of Chromatography B: Biomedical Sciences and Applications
Disclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
