Abstract
Purpose:Bloodstream infections are a general cause of death among hospitalized patients. Rapid diagnosis and timely treatment can reduce mortality. The aim of this investigation was to evaluate the 23S rRNA PCR assays as a rapid detection method for diagnose of sepsis in patients with suspected bacteremia.Methods:A cross-sectional study was conducted at Shahid Beheshti University Hospital in Kashan from November 2017 to December 2018. The blood samples of 265 patients with suspected bacteremia were studied by blood culture and 23S rRNA PCR techniques. The results were analyzed using SPSS version 16 and Chi-square test.Results:Eighty (30.2%) blood samples of 265 suspected patients, were identified as positive by PCR assays, whereas 27 (10.2%) were identified as positive by the blood culture technique. The statistical analysis showed a significant association between the results of PCR assays and blood culture and factors such as prior antibiotic use and underlying diseases (P˂0.05). Also a significant correlation was observed between laboratory and clinical criteria and the results of both PCR assays and blood culture (P˂ 0.05).Conclusion:The 23S rRNA PCR method is a rapid and sensitive technique specially for diagnosing sepsis among patients in whom bacteremia is difficult to diagnose with culture method including neonates and patients who have taken antibiotics before microbial culture.
Highlights
Bloodstream Infections (BSIs) are the second cause of death among patients who are hospitalized in the Intensive Care Unit (ICU) [1]
The PCR assays using 23S rRNA genes identified 80 (30.2%) of all patients with BSIs
3 Acinetobacter baumannii, and 1 Klebsiella pneumoniae) whereas 3 blood culture positive samples were PCR negative which were identified as coagulase-negative staphylococci
Summary
Bloodstream Infections (BSIs) are the second cause of death among patients who are hospitalized in the Intensive Care Unit (ICU) [1]. The mortality rates due to blood stream infections vary in different parts of the world and range from. Despite the fact that sepsis was known 2000 years ago, but the definition of it is still challenging for clinicians and there is no 'gold standard' in this context. For evaluation of the intensity of sepsis, a reliable method is needed. The availability of standard guidance helps clinicians to better manage patients and increase patient survival [5]. The presence of an infection and two or more Systemic Inflammatory Response Syndrome (SIRS) criteria including
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