Rapid Detection of Glucose on Nanostructured Gold Film Biosensor by Surface-Enhanced Raman Spectroscopy.

Cheng-Ju Sung,Shih-Chieh Hsu,Szu-Han Chao

doi:10.3390/bios11020054

Cheng-Ju Sung, Shih-Chieh Hsu + Show 1 more

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https://doi.org/10.3390/bios11020054

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Journal: Biosensors	Publication Date: Feb 19, 2021
Citations: 7	License type: CC BY 4.0

Affiliation: Tamkang University

Abstract

In this report, we summarized our development of biosensors for Rhodamine 6G and in vitro glucose detection based on surface-enhanced Raman scattering technology. For the detection of both Rhodamine 6G and in vitro glucose, a nature-patterned substrate with gold films over nanostructures (NPS-AuFON) was used as the surface-enhanced Raman scattering sensor platform. The enhancement factor was calculated at 9 × 107. In the processing of the substrate, cyclic voltammetry was used to form nano-gold particles under different conditions. The Rhodamine 6G and glucose detection were then achieved on this substrate. Furthermore, we combined the potentiostatic technique and electrochemical adsorption to best detect glucose in low concentrations. The glucose oxidation potential (100 mV) was used to capture glucose close to the surface of the NPS-AuFON. The quantitative detection of glucose in solution and in situ inspection were confirmed. Further, we determined that this surface modification technology can reach the goal of experiments set by the World Health Organization to judge whether or not a patient is a diabetic by detecting a glucose concentration of 11.1 mmol/L (mg/dL) at a minimum.

Highlights

Introduction on Nanostructured Gold FilmIn 1974, Fleischmann et al [1] added pyridine molecules to 0.1 M of potassium chloride electrolyte to perform several redox reactions on a silver electrode
Different concentrations of sulfuric acid (0.1 M, 0.25 M, and 0.5 M) and one needs to understand the behavior of element deposition and examine the composition different cyclic voltammetry (CV) (0, 3, 6, and 9) with a fixed sweep rate (10 mV/sec) were used to change the of the surface of the surface-enhanced Raman scattering (SERS) substrate
The Raman indifferent CVs (0, 3, 6, and 9) with a fixed sweep rate (10 mV/sec) were used to change the tensity and signal-to-noise ratio (S/N ratio) of the Raman peak was even better than the surface roughness for enhancing the surface plasmon resonance to achieve the SERS effect

Summary

Introduction

Introduction on Nanostructured Gold FilmIn 1974, Fleischmann et al [1] added pyridine molecules to 0.1 M of potassium chloride electrolyte to perform several redox reactions on a silver electrode. They speculated that this was caused by the redox treatment performed on the silver electrode in the experiment which caused the surface roughness of the silver electrode to increase, and subsequently it adsorbed more pyridine molecules They found that when the number of pyridines increased, the overall Raman scattering intensity increased, resulting in the observation of the Raman signal in a single layer of molecules. In 1977, Van Duyne et al studied the adsorbed pyridine on a silver surface and extended its applicability to other nitrogen heterocycles and amines [2] They found that the intensity of the Raman spectrum increased by 105 and claimed that redox could not fully explain the increase of the Raman signal, they mentioned the concept of an adsorption enhancement effect.

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