Abstract
We present a new “Rapid Catch and Signal” (DNA-RCS) technology for the simultaneous highly selective detection of multiple DNA sequences in solution. The DNA-RCS technology combines the advantages of microwave accelerated DNA hybridization on surfaces, i.e. the simultaneous recognition of several target-DNA sequences in one sample well by complementary anchor-DNAs, attached to a silver-island film (SiF), (rapid catch) and the Metal-Enhanced Fluorescence (MEF) technology (signal). Fluorescent DNA labels, which play the role of molecular sensor probes, show a strong response on DNA-DNA recognition events due to fluorophore coupling with nanoparticle plasmons at short (10 - 30 nm) distances from the surface. Microwave-accelerated DNA hybridization on the SiF-surface subsequently brings the sensor-DNA probes close to the silver-nanoparticles, significantly enhancing the fluorescence signal, a technology described by Geddes as “Metal-Enhanced Fluorescence” (MEF). Subsequently the multi-color RCS-DNA assay platform can radically expedite quantitative analysis of genome DNA sequences within ∼20 seconds at very high sensitivity with significant advantages over the widely used PCR approach.
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