Abstract
The rapid separation of sodium dodecyl sulphate-protein complexes according to their molecular masses ( M r) by capillary gel electrophoresis is described. Using commercial equipment, standard proteins with M r in the range 29 000–97 400 were resolved to the baseline in less than 2 min by utilizing a separation distance of 7 cm. A linear relationship between migration time and log M r was found and rapid determination of the molecular mass of light and heavy chains of human immunoglobulin G is reported. The results are compared with applications using longer separation distances, showing that rapid and efficient analysis and adequate resolution can be obtained by using short separation distances.
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