RAPID AND SIMPLE QUANTITATIVE DETERMINATION OF ATRACTYLENOLIDE I AND ATRACTYLENOLIDE III IN ATRACTYLODES MACROCEPHALA AND ITS DIFFERENT PROCESSED PRODUCTS BY CAPILLARY ZONE ELECTROPHORESIS

Abstract

Atractylenolide I (AO-I) and atractylenolide III (AO-III) are the major sesquiterpenes considered to be bioactive from Atractylodes macrocephala (RAM) and its different processed products, popularly used for the treatment of the various digestive diseases and tumors. A rapid and simple capillary zone electrophoresis (CZE) method to quantitatively determine the AO-I and AO-III is described. Using an optimized method, AO-I and AO-III were separated in less than 10 min with A 50 mM borate buffer and a separated voltage 20 kV at 25°C. The CZE method was validated for linearity, sensitivity, accuracy, and precision and then used to determine the content of AO-I and AO-III. This method was successfully applied to determine AO-I and AO-III in real sample RAM and its different processed products.