Abstract
Non-polar lipids were separated by high-performance thin-layer chromatography on silica gel plates and detected by use of a new reagent that induced fluorescence in the separated components. Developed thin-layer plates are dipped into a solution of sulfuric acid—ethanol—hexane (1:35:64, v/v), heated and the lipid classes are quantified by fluorescence densitometry. This technique allowed detection of certain standard lipids at the 5-ng level, is well suited for the rapid and efficient analysis of large numbers of samples and offers distinct advantages over other in situ fluorescence inducing methods. The method was successfully applied to the analysis of the non-polar lipids that occur in enzymatically hydrolyzed beef tallow.
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
