Abstract
Muramic acid was analysed by high-performance liquid chromatography with precolumn derivatization using o-phthalaldehyde in a standard solution and in a culture of marine bacteria from a natural sample. The effects of buffer pH were studied in order to optimize the separation of muramic acid from interfering amino acids. A linear relationship was found between the fluorescence response and muramic acid concentration. The muramic acid-( o-phthalaldehyde) derivative gave a single sharp peak, and complete separation from interfering amino acids was achieved at the picomole level in a sort time (3 h for preparation and 10 min for chromatography with the bacterial sample).
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