Rapid and sensitive detection of dextran sulfate sodium based on supramolecular self-assembly of a perylene diimide derivative in aqueous solution

Abstract

Dextran sulfate sodium (DSS) is a heparin polysaccharide, whose overuse would cause many adverse effects, such as stiff and painful joints and loss of hair. It is crucial to search after a rapid and accurate approach for the determination of DSS in the clinical treatment and diagnosis. Regrettably, there are few literatures about analytical methods for detecting DSS at present. In this research, a sensitive and selective method for detecting DSS based on the hydrosoluble perylene diimide (PDI) derivative was established in pure aqueous solution. The sensing mechanism and performance of PDI-PdEC were systematically investigated by ultraviolet and fluorescence spectroscopy. Non-covalent interactions between DSS and PDI-PdEC, like π-π stacking, electrostatic and hydrophobic interaction, promote the aggregation of PDI-PdEC and form supramolecular aggregates, which realize the sensitive and rapid detection of DSS in aqueous system. The detection limit for DSS is as low as 5.51 ng/mL and the linear range is 20–500 ng/mL. Furthermore, this probe was triumphantly applied in the detecting of DSS in serum. To the best of our knowledge, this is the first fluorescent probe for detecting DSS in aqueous media. We consider that our study will not only broaden the range of applications of perylene diimide fluorescent sensors, but also provide valuable reference for the design of new sensors for the rapid determination method of DSS.