Rapid and Label-Free Electrochemical DNA Biosensor for Detecting Hepatitis A Virus

Abstract

first_page settings Order Article Reprints Font Type: Arial Georgia Verdana Font Size: Aa Aa Aa Line Spacing:    Column Width:    Background: Open AccessAbstract Rapid and Label-Free Electrochemical DNA Biosensor for Detecting Hepatitis A Virus † by Marisa Manzano 1, Sara Viezzi 1, Sandra Mazerat 2, Robert Marks 3 and Jasmina VIDIC 4,* 1 Dipartimento di Scienze Agroalimentari, Ambientali e Animali, University of Udine, 33100 Udine, Italy 2 Institut de Chimie Moléculaire et des Matériaux d’Orsay Chimie Inorganique, Paris Saclay University, 91405 Orsay, France 3 Department of Biotechnology Engineering, Ben-Gurion University of the Negev, Beer-Sheva 8410501, Israel 4 Virologie et Immunologie Moléculaires, Paris Saclay University, INRA, 78350 Jouy en Josas, France * Author to whom correspondence should be addressed. † Presented at the 5th International Symposium on Sensor Science (I3S 2017), Barcelona, Spain, 27–29 September 2017. Proceedings 2017, 1(8), 794; https://doi.org/10.3390/proceedings1080794 Published: 11 December 2017 (This article belongs to the Proceedings of Proceedings of the 5th International Symposium on Sensor Science (I3S 2017)) Download Download PDF Download PDF with Cover Download XML Versions Notes The hepatitis A virus (HAV) presents one of the most important foodborne pathogens causing a worldwide health problem each year. The control of HAV outbreaks remains difficult as conventional PCR-based methods fail to detect low levels of the virus in water and foods. We developed a highly sensitive and specific analytical method for detection of HAV. The device comprises a thiol-terminated DNA probe, complementary to the specific HAV sequence, embedded onto a gold electrode where the DNA hybridization is a sensing mechanism. The electrochemical measurements demonstrated that this device detected HAV DNA template over a wide concentration range from 10 fg/µL to 1 ng/µL with the calculated limit of detection of 0.398 fg/µL for the complementary ssDNA sequence and 3.2 fg/µL for viral cDNA obtained by PCR, respectively. The DNA-sensor developed can be potentially adopted as an easy-to-use and low cost method for screening HAV in contaminated food samples. Conflicts of InterestThe authors declare no conflict of interest.Publisher’s Note: MDPI stays neutral with regard to jurisdictional claims in published maps and institutional affiliations. © 2017 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). Share and Cite MDPI and ACS Style Manzano, M.; Viezzi, S.; Mazerat, S.; Marks, R.; VIDIC, J. Rapid and Label-Free Electrochemical DNA Biosensor for Detecting Hepatitis A Virus. Proceedings 2017, 1, 794. https://doi.org/10.3390/proceedings1080794 AMA Style Manzano M, Viezzi S, Mazerat S, Marks R, VIDIC J. Rapid and Label-Free Electrochemical DNA Biosensor for Detecting Hepatitis A Virus. Proceedings. 2017; 1(8):794. https://doi.org/10.3390/proceedings1080794 Chicago/Turabian Style Manzano, Marisa, Sara Viezzi, Sandra Mazerat, Robert Marks, and Jasmina VIDIC. 2017. "Rapid and Label-Free Electrochemical DNA Biosensor for Detecting Hepatitis A Virus" Proceedings 1, no. 8: 794. https://doi.org/10.3390/proceedings1080794 Find Other Styles Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here. Article Metrics No No Article Access Statistics Multiple requests from the same IP address are counted as one view.