Abstract
The genetic variability and relationships among 5 Egyptian wheat genotypes representing Sakha8, Sakha69, Sakha93, Sids1 and Gemmiza7 were analyzed using 8 random amplified polymorphic DNA (RAPD). A total of 77 loci (73 % polymorphic) in all 5 wheat genotypes was amplified and discriminated all the wheat genotypes. PIC, RP, MI, DP values were evaluated and revealed degree of genetic divergence among the cultivars used. A cluster based on UPGMA (Un-weighted Pair-Group Method with Arithmetic Mean) analysis was used to determine genetic similarities. The five wheat genotypes were divided into two main clusters. Cluster 1 was divided into two groups. In subgroup 1 were included genotype 1 and genotype 2. They seemed very close which might depict sharing of the genetic background among the genotypes. In consequence, the close genetic relationships are entirely alarming and may hinder further plant improvement. Genotype 5 was in subgroup 2. The second cluster was included genotype 3 and genotype 4. The same genotypes were also assessed in field conditions for structural analyses, which were carried out based on six yield components. The dendrogram created was comparatively analyzed with the RAPD dendrogram. This study additionally indicates that RAPD markers are useful for distinguishing and characterizing wheat cultivars. The genetic relatedness among these genotypes could provide useful information for conservation and selection of cross parents in breeding.
Highlights
Wheat (Triticum aestivum L.) is one of the most important and widely cultivated crops in the world, used mainly for human consumption and support nearly 35% of the world population
The level of polymorphism observed was high (73%), indicating a wide and diverse genetic based for genotypes used
According to polymorphism information content (PIC), Resolving power (RP), Marker index (MI), Discrimination power (DP) values, random amplified polymorphic DNA (RAPD) primers seem to be the suitable technique for characterization the wheat varieties
Summary
Wheat (Triticum aestivum L.) is one of the most important and widely cultivated crops in the world, used mainly for human consumption and support nearly 35% of the world population. The total cultivated area of wheat is more than 200 million hectares and total wheat production is about 733 million tons per year [1]. By 2020, the world demand for wheat is expected to be 40% higher than that of its level in the latter half of the 1990s [2]. Wheat is self-pollinated, hexaploid crop which having 2n= 6x= 42 chromosome. The hexaploid wheat have large genome size (approximately 17,000 Mb), a high proportion of repetitive DNAs, continuous inbreeding caused by self-pollination and a narrow genetic base represent the difficulties for use of molecular markers [4]
Sign-in/Register to view highlights & summary Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
