Assessment of stability and viability of a jute hybrid (Corchorus capsularis L. × C. trilocularis L.) and its trueness based on RAPD markers

Abstract

A hybrid line (Corchorus capsularis L. – pollen parent × C. trilocularis L. – stigma parent) of jute (family: Tiliaceae) has been assessed for its stability (using cytomorphological parameters) and viability (using different stain tests including DAPI - 4′,6-diamidino-2-phenylindole) in advance generations (F4 to F6). The trueness of the hybrid is studied following the use of RAPD (random amplification of polymorphic DNA) markers. Results indicate that the hybrid line is stable, viable and true. UPGMA (Unweighted Pair Group Method with Arithmetic Mean) analysis suggests a close relationship as well as distinctiveness between parents and the raised hybrid. Upon considering different genetic efficiency parameters, taken together, it seems that OPA 02, OPA 03, OPB 01, OPB 02, OPB 06, OPC 01 and OPC 06 are effective and efficient RAPD markers for molecular screening of jute germplasms including hybrid(s). OPB 06 is found to be female specific RAPD marker.