Abstract
Eight isolates of Sclerotium rolfsii from four strategically geographical sites of Bangladesh were characterized and their cultural properties like average linear mycelial growth, colony colour, colony consistency, growth pattern and sclerotia formation were studied. Isolates varied in mycelial growth and other growth characteristics and were grouped into three. The highest linear growth was displayed by S8. DNA concentration of eight isolates varied from 1150-7200 ng/?l. DNA fingerprinting by RAPD prompted the grouping of isolates. Selected 3 primers generated 20 bands with size ranging from 100-1500 bp. Out of the 20 bands, 9 bands (45%) were polymorphic and 11 bands (55%) were monomorphic among the eight isolates of Sclerotium rolfsii. The co-efficient of gene differentiation (Gst) was 1.000 reflecting the existence of high level of genetic variations among the 8 isolates. The lowest genetic distance and highest inter isolate similarity was found in S1 and S2 which would be homogeneous. The highest genetic distance and lowest inter isolate similarity found in S3, S7 and S3, S8 pair which would be most divergent isolates. The cluster analysis also revealed that S3, S7 and S8 belong to different clusters. All five varieties of eggplant and tomatoes were graded as susceptible when inoculated with eight isolates. Plant mortality 93.33% was recorded in S4, S6 and in S8. Considering the isolate factor the most virulent isolate would be S8 whereas the less virulent isolate would be S2 and S7. Host plant of S8 was tomato collected from Thakurgaon. S2 and S7 were collected from BAU farm and Dinajpur and host plants were lentil and tomato respectively. It is evident that Sclerotium rolfsii from Thakurgaon on host tomato is more virulent.Int. J. Agril. Res. Innov. & Tech. 6 (1): 47-57, June, 2016
Highlights
Sclerotium rolfsii Sacc. is a soil borne plant pathogen causing diseases on a wide range of agricultural and horticultural crops
We have studied the morphological feature of the fungus
The highest mycelial growth was observed in S8 (8.633) followed by S5, S7, S1 and S4
Summary
Sclerotium rolfsii Sacc. is a soil borne plant pathogen causing diseases on a wide range of agricultural and horticultural crops. Is a soil borne plant pathogen causing diseases on a wide range of agricultural and horticultural crops It has wide geographic diversity and commonly found in the tropics, subtropics and other warm temperate regions especially in the Southern United States, Central and South America, West Indies, Southern European countries bordering the Mediterranean, Africa, India, Japan, Philippines and Hawaii (Aycock, 1966) and in warmer region like Bangladesh (Talukder, 1974; Ahmed and Hossain, 1985).The wide host range, prolific growth and ability to produce persistent sclerotia contribute to the large economic losses associated with this pathogen. The disease foot/collar rot causes 60-100% death of eggplants (Siddique et al, 2002)
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