Abstract
Ninety six lentil accessions from different origins were collected from National Grain Legume Research Program, Rampur; Regional Agriculture Research Station, Nepalgunj and National Agriculture Genetic Resource Center, Khumaltar, Lalitpur. Among them; four lines were Nepal Local, forty two lines were Nepal Cross; forty seven lines were ICARDA Line and finally three lines were Indian Line. All ninety six accessions were analysed by DNA fingerprinting using thirty three selected polymorphic SSR markers. The characterization was performed in Biotechnology Unit, Nepal Agricultural Research Council, Khumaltar, Lalitpur by using standard protocols. Molecular variance analysis showed that 14 % genetic variation was found between population and 86 % genetic variation was found within population with estimated variance 0.23 between population and 1.35 within population. Highest genetic distance (9) was found between landrace ILL-7979 and RL-20. In the same way, highest Nei genetic distance (0.03) between population was shown by population 1 and population 4; and lowest genetic distance were observed within the same population accessions. The heterozygosity was probably due to the introgression of genes or duplication of microsatellite motif during the breeding and or the course of lentil line evolution. All the accessions included in this study displayed significant amount of genetic variability and genetic relatedness due to different center of origin and different genetic constitutions. The diversity detected in this study may constitute the new materials for future systematic lentil breeding programs.DOI: http://dx.doi.org/10.3126/ijasbt.v1i3.8608 Int J Appl Sci Biotechnol, Vol. 1(3) 2013 : 132-136
Highlights
The knowledge of genetic diversity and association of characters with yield is of great importance to the breeder for making an improvement of quantitative characters
High genetic relatedness were found within the same population which might be due to same center of origin and similar genetic constitutions
In the same way high genetic distance were found between Nepal Line and Indian Line which might be due to different center of origin and different genetic constitutions
Summary
The knowledge of genetic diversity and association of characters with yield is of great importance to the breeder for making an improvement of quantitative characters. Molecular marker is used for estimating genetic variation at population level and among closely related species (Nienhuis et al, 1995). Several classes of molecular markers have been developed showing that lentil has relatively low levels of genetic variation (Eujay let al., 1997; Sonnante and Pignone, 2001). Nepal has ha area 1,87,437altogether of lentil 1,51,758with d per hectare kg yiel 810metric ton productivity and M)oAD .(2011 ,The crop has developed into a range of varieties adapted to diverse growing areas and cultural preferences, and containing unique nutritional compositions, colors, shapes and tastes. A lot of lentil land races, primitive races, indigenous races and wild races are still available in Nepal but they have not been studied properly .The genetic relatedness of lentil based on molecular level has not been studied yet in Nepal. Present study was conducted with an objective of selecting divergent parents based on genetic distance for future lentil breeding programme
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