Raman and FT-IR Spectroscopy investigation the cellulose structural differences from bacteria Gluconacetobacter sucrofermentans during the different regimes of cultivation on a molasses media

Nelli Atykyan,Victor Revin,Vitalina Shutova

doi:10.1186/s13568-020-01020-8

Abstract

Raman and Fourier Transform Infrared (FT-IR) spectroscopy was used for investigation of structural differences of bacterial celluloses (BC), obtained by cultivation native and immobilized cells of Gluconacetobacter sucrofermentans during static and dynamic regimes of cultivation on a molasses media. It was found that the Raman and FT-IR spectra could characterized the groups of the cellulose molecules. The culturing bacterial cellulose in the presence of results in an increase of crystalline and it increased during cultivated on a molasses media with the addition of 1.5% ethanol—75.62%. The degree of BC crystallinity increased during dynamic regime of cultivation is higher than under static regime one. The maximal BC content was observed when 0.5% ascorbic acid was added to the cultivation medium with molasses and native cells. It was found, the degree of BC crystallinity during static regime cultivation on a molasses medium with ethanol, increased significantly to 73.5%, and during dynamic regime—75.6%. So, in this study, the changes of the bacterial cellulose conformation of were revealed during bacterial cultivation in a medium containing molasses in various cultivation modes.

Highlights

Bacterial cellulose (BC) is carbohydrates produced by Komagataeibacter, Gluconacetobacter, Enterobacter, Pseudomonas, Achromobacter, Alcaligenes, Aerobacter, Azotobacter, Agrobacterium, Burkholderia, Dickeya, Escherichia, Rhizobium, Salmonella, and Sarcina with unique biotechnological properties and wide range of applications (Klemm et al 2001; Zhu et al 2001; Maneerung et al 2008; Cauerhff and Castro 2013; Lee et al 2014; Augimeri et al 2015; Cacicedo et al 2015; Rastogi et al.2018)
2011); (3) glucose and sucrose are used for bacterial celluloses (BC) synthesis, but fructose, maltose, xylose, starch, glycerin, ethanol and other sources of carbon can be used (Hestrin and Schramm 1954; Park et al 2003; Hungund et al 2013)
The latter is important because the plant celluloses and BC have the same molecular structure, but differ in crystalline structures connected with its crystalline structure polymorphs: in nature; cellulose

Summary

Introduction

Bacterial cellulose (BC) is carbohydrates produced by Komagataeibacter, Gluconacetobacter, Enterobacter, Pseudomonas, Achromobacter, Alcaligenes, Aerobacter, Azotobacter, Agrobacterium, Burkholderia, Dickeya, Escherichia, Rhizobium, Salmonella, and Sarcina with unique biotechnological properties (the finest porosity and mechanical strength) and wide range of applications (promising material for medicine, industry and technology) (Klemm et al 2001; Zhu et al 2001; Maneerung et al 2008; Cauerhff and Castro 2013; Lee et al 2014; Augimeri et al 2015; Cacicedo et al 2015; Rastogi et al.2018). Concerning, the carbon sources are often the most expensive components of the culture medium, so modern biotechnology offers to use sugar-containing waste from food industries, such as molasses, DDGS, whey (Keshk and Sameshima 2006; Kongruang 2008; Coban and Biyik 2011; Zeng et al 2011; Guo et al 2013; Rani and Appaiah 2013; Wu and Liu 2013; Jozala et al 2014; Lin et al 2014; Kiziltas et al 2015). The crystalline domains in BC are of very limited size and are mixed with noncrystalline regions The latter is important because the plant celluloses and BC have the same molecular structure, but differ in crystalline structures connected with its crystalline structure polymorphs (cellulose I–IV): in nature (cellulose I); cellulose

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