Radish Juice Promote Kidney Stone Deposition in Ethylene Glycol-induced Urolithiasis in Rats

Abstract

Urolithiasis is a well-known problem that stones could form in various parts of the urinary system and it is the most common disease of the urinary tract. The current study was planned to investigate the effect of radish juice on ethylene glycol (EG)-induced urolithiasis. Twenty-one rats randomly divided into three groups. The first group was the control group was received normal standard diet and drinking water and the second group represented the model group received 0.75% EG in drinking water ad libitum. The third group received radish juice (2 ml/kg of body weight) by gavage plus EG (0.75%) in drinking water ad libitum. The experiment was conducted for 28 days. The light microscope examination revealed a disturbed histological architecture of the kidney tissues, including dilated renal tubules, aggregation of infiltrated leukocytes inflammatory cells, and crystal deposition in the model group. The EG plus radish juice treated rats showed higher crystal density with improved renal tubule structure and alleviated inflammation. Both treated groups showed various biochemical alterations compared to control group, but the most interest biochemical result was the significant decrease of malondialdehyde, a lipid peroxidation marker, and in the radish plus EG group compared to the EG group. Scanning electron microscopy showed clear structural detail about calcium oxalate crystals in which radish-treated group showed higher crystal deposition and calcified tissue compared to EG group. The present study concluded that radish juice promotes stone deposition but exerted an antioxidant effect.