Abstract
BackgroundIn spite of advances in the modern allopathic medicines, there is no satisfactory treatment of kidney stones, so formation and growth of calculi continues to trouble mankind. In India, many herbal formulations are in use for the treatment of urolithiasis. The purpose of the present study was to investigate the antiurolithiatic efficacy of combined extract of plants Dolichos biflorus (D.b) (hydroalcoholic seed extract) and Crataeva nurvala (C.n) (aqueous bark extract) in ethylene glycol-induced urolithiasis in Wistar rats. Rats were divided into 4 groups. Ethylene glycol (0.75% v/v, p.o.) was administered for 35 days. Different drug treatments were given from the 21st to 35th day of the study. On the last day, rats were sacrificed, and different samples were taken for further analysis.ResultsBoth the combination drug treatments were found to be effective in treating urolithiasis. More significant protection was observed on treatment with the fraction ratio of D.b + C.n (3:1). Histopathology analysis showed degenerated glomeruli and inflammatory cells in urolithiasis control. The same were regenerated on treatment with combined extract of the two plants.ConclusionAdministration of the combined plant extracts in a ratio of D.b + C.n (3:1) possesses better efficacy against ethylene glycol-induced urolithiasis in rats which may be evaluated further for mechanistic pathway elucidation in vivo.
Highlights
In spite of advances in the modern allopathic medicines, there is no satisfactory treatment of kidney stones, so formation and growth of calculi continues to trouble mankind
Preliminary phytochemical screening The results of phytochemical screening showed the presence of a number of secondary metabolites including carbohydrates, alkaloids, proteins, and flavonoids (Table 1)
Ratio 3:1 of D.b + C.n (9.2 ± 0.03 mg/dl) showed better results than ratio 1:1 (11.94 ± 0.08 mg/dl) and were comparable to the standard group (8.12 ± 0.05 mg/dl). These results indicate that the combination of the two test drugs shows more effective protection when the concentration of D.b is more, i.e., in the ratio of D.b + C.n (3:1) against ethylene glycol-induced urolithiasis
Summary
In spite of advances in the modern allopathic medicines, there is no satisfactory treatment of kidney stones, so formation and growth of calculi continues to trouble mankind. There are few recent reports of beneficial effects of medical treatments in enhancing clearance of calculi in the distal ureters [7], no Kaushik et al Future Journal of Pharmaceutical Sciences (2021) 7:21 promising antiurolithiatic agent has been reported, especially for the prevention of the recurrence of stones. In this regard, many plants have been conventionally used to treat kidney calculi and have been shown to be effective. Schult. [9], Moringa oleifera Lam. [10], Asparagus racemosus Willd. [11], Rotula aquatica Lour. [12], Cyclea peltata (Lam.) Hook F. & Thoms [11], Tribulus terrestris L. [13], Musa sapienta L. [14], Ammannia baccifera L. [15], Mimosa pudica L. [16], Crataeva nurvala Buch-Ham. [17], etc
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Free) 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call
