Abstract
A radiotracer enzyme assay for the determination of pyruvate kinase activity is described. The rate of the enzyme catalyzed reaction is determined by radioassay of either ATP- 3H formed or unreacted ADP- 3H, or both after their separations from the assay reaction mixture by TLC. The method is extremely sensitive, permitting the use of microgram quantities of tissue to assay for the enzymic formation of μμmole quantities of product.
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