Abstract
A fluorometric technique for the determination of acid-soluble phosphorus, lipid phosphorus, and nucleic acid phosphorus in 0.5 to 1.0 μg quantities of tissue is described. The assay is based on the stoichiometric generation of NADPH from P i by the use of purified crystalline enzymes, a feature which confers the advantage of extension to even greater sensitivity by application of the “NADPH cycling” system. Assays of mouse brain and mouse brain tumor were performed at both the microgram and milligram level and compared. The fluorometric assay offers about fivefold increase in sensitivity with a sacrifice of analytical accuracy of approximately 6% (4% vs. 10%) when compared to an established spectrophotometric procedure.
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