Radiotherapy Radio protective effect of 2- deoxy D-glucose with combination of selective antioxidant medicinal plant Vitis vinifera, Camellia sinensis, and Zingiber officinale extracts in mice

Abstract

High doses of radiation therapy are used to destroy cancer cells, these high doses of radiations also damage healthy cells and tissues near the treatment area or whole body. At present, major advances of radiation therapy have made it more precise, leading to fewer side effects. The side effects often start during second week of radiation treatment So it is necessary to minimize these side effects with natural available medication. Previous studies shown that, 2- deoxy D- glucose, an analogue of glucose and glycolytic inhibitor, enhances radiation induced damage, selectively in tumour cells while protecting normal cells suggest that 2- deoxy D- glucose can be used as a differential radio modifier to improve the efficacy of radiotherapy and also it is shown that antioxidant medicinal plants like vitis Vinifera, camellia Sinensis, and zingiber officinale have shown protective effect against radiation induced damage in previous studies. So in the present study, we have selected these antioxidant medicinal plant extracts combines with 2- deoxy D- glucose as a treatment, before and after radiation therapy and measured the levels of nitric oxide, lipid peroxidation and antioxidant enzymes like superoxide dismutase, catalase and glutathione peroxidase in tissues like blood, liver, kidney and brain tissues of mice after completion of study. In the present study, it is found that the highest effect is found in a group of animals which are on combination of normal diet and feeding of deoxy – D glucose with grape seed extract, green tea extract and ginger extract in proportion of 1:1:1, in this group of animal’s radiation induced micronucleus formation, formation of metamyelocytes and dysplastic myeloid series cells formation is also found significantly decreased.