Probiotic, bacillus amyloliquefaciens from milk sample of a native cow ‘Kangeyam’ breed in forest fringe village of Coimbatore, Tamil Nadu and value addition thereof

Abstract

Probiotics include good microorganisms that support the body's ability to operate and stay healthy. These good bacteria have several advantages, including as helping us feel better and warding off bad bacteria when there are too many of them in the stomach. Milk samples from the native indigenous Kangeyam breed cattle reared in the tribal hamlets on the forest fringes of Coimbatore were collected to isolate the beneficial microorganism. The sample is specifically collected from such region as it might have a higher efficiency in comparison with domesticated livestock. The milk samples collected were processed and analyzed for their nutritional value. Milk samples obtained from the native indigenous Kangeyam breed cattle reared in tribal hamlets of forest fringe villages was found to be more efficient in the nutritional aspects as like the difference in the milk from regular domesticated cattle. The quality of the milk was tested and then the probiotics were isolated. The pure culture was obtained in which the basic confirmatory tests such as Gram staining, Motility, IMViC, Catalase, Oxidase, Urease, Gas from glucose, Triple sugar iron test, etc., were done. Culture with more accurate results was made for molecular identification by 16srRNA method, in which the species Bacillus amyloliquefaciens has been confirmed and submitted to NCBI. The probiotic was found to be beneficial. The safety assessment was studied for accepted intake. Then the culture was made frozen, which was further lyophilized to be made into powder. The isolated probiotic with significant health benefits can be considered as a probiotic infused value-added product with commercialization potential.