Abstract
A new method is presented for the on-line detection of beta-labelled compounds of high polarity in reversed-phase column liquid chromatography. The aqueous column effluent containing the 14C-labelled amino acids alanine, valine, isoleucine and/or leucine is segmented with a non-water-miscible liquid scintillator. This two-phase system is introduced into the beta-detector without phase separation, where the betas emitted in the aqueous phase are detected in the scintillator phase. The detector effluent is collected in a storage capillary tube. At the end of the chromatographic process, the contents of the storage capillary tube are introduced into the beta-detector by reversing the direction of the flow. The two phases are homogenized by adding a water-miscible liquid scintillator. By lowering the flow-rates the signal increases, depending on the flow-rates used.
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