Abstract
Amino acid transport in Lactobacillus helveticus was analyzed. Strain specificity of amino acid transport was speculated between L. helveticus NCDO2712 and SBT2171. Glucose energized L. helveticus NCDO2712 actively transported and accumulated the essential and growth stimulating amino acids (leucine, isoleucine, valine, threonine, lysine, aspartic acid, glutamic acid, tyrosine, arginine, and histidine). Uptake of proline, phenylalanine and tryptophan was not observed. Transport studies in isolated membrane vesicles of L. helveticus fused with liposomes to reduce the passive proton permeability revealed that amino acids leucine, isoleucine, valine, threonine, and lysine are transported by a proton motive force coupled system. These results indicated that L. helveticus and Lactococcus lactis amino acid transport systems are similar.
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