Abstract
ABSTRACT A reliable and sensitive radioimmunoassay for human growth hormone has been devised in which separation of antibody-bound and free radiohormone (HGH-125I) is accomplished by the principle of enzyme partition. Since the proteolytic enzyme, ficin, rapidly destroys most free HGH-125I, but does not degrade antibody-bound radiohormone, the 2 fractions can be readily distinguished by precipitation of the antibody-bound HGH-125I with trichloroacetic acid solution. With this elementary system, endogenous levels of growth hormone in serum can be conveniently measured in less than 24 hr with results that are comparable in precision and sensitivity with those of longer techniques that are currently in use. The successful application of enzyme partition to the assay of growth hormone, and previously to the measurement of insulin, suggests that the same principle could be employed to assay other hormones or substances that are bound by specific antibodies or nonspecific binding substitutes.
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