Abstract
Abstract A specific radiochemical microassay for δ-aminolevulinic acid synthetase is described. The assay is rapid and 10 to 500 times more sensitive than currently available methods. The assay measures the incorporation of radioactive succinate into δ-aminolevulinic acid and requires a succinyl CoA generating system; alternate pathways of succinate utilization are blocked by specific metabolic inhibitors. The δ-aminolevulinic acid formed is isolated by ion-exchange chromatography. The increased sensitivity of this assay has permitted the direct measurement of δ-aminolevulinic acid synthetase activity in cell cultures of avian hepatocytes, needle biopsy specimens of human liver, peripheral erythrocytes, and human fibroblasts.
Published Version
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