Radical Scavenging Linked Antioxidant Comparison and Quantification of Conventional and Supercritical Fluid Ginger Extracts

Abstract

Phytoceutics have been become increasingly famous with individuals anxious to mitigate the influence of unhealthy lifestyle as well as aging. Purposely, phytochemical analysis of ginger proved it as an excellent source of antioxidants and free radical scavengers. Regarding bioactive moieties especially gingerol and shogaol series extraction, ethanol showed maximum radical scavenging at 90 minutes than that of ethyl acetate and acetone. Ginger conventional extract showed maximum activity as 57.80 ± 1.97% superoxide anion radical scavenging, 38.23 ± 1.30% nitric oxide radical scavenging, 77.62 ± 2.64% hydroxyl radical scavenging and 52.61 ± 1.79% hydrogen peroxide scavenging activity in ethanol extract at 90 minute. Regarding to supercritical fluid extracts the maximum activity was observed at 3300 psi pressure and 40°C for 2 hr, followed by 3600 psi and 3000 psi pressure at same conditions. The maximum values were 78.13 ± 2.50%, 54.83 ± 1.75%, 86.71 ± 2.81% and 75.42 ± 2.26% for superoxide, nitric oxide, hydroxyl radical and hydrogen peroxide radical scavenging activity of supercritical fluid ginger extract. Afterwards, HPLC analysis portray that ginger extracts contain maximum amount of in gingerol (7.81 mg/g) at 3300 psi pressure followed by 3600 psi and 3000 psi pressure for supercritical and 5.74 mg/g in ethanolic extract among organic solvents.