Radical-Scavenging activities of total extracts and identification of four anthocyanin isomers from two purple-fleshed varieties of Ipomoea batatas L. produced in Burkina Faso, by hplc-ms/ms

Abstract

Anthocyanins are bioactive compounds which, due to their free radical scavenging properties, can protect the human body against oxidative stress that can cause many diseases, such as cancer, aging. The Tuskegee purple Cap Vert and Tuskegee purple sweet potato (Ipomoea batatas L.) varieties are rich in anthocyanins. Indeed, their total anthocyanin contents are estimated at 0.211 and 0.122 mg/g of fresh plant material, respectively. And the antiradical activities of the varieties Tuskegee purple and Tuskegee purple Cap Vert extracts were estimated at 0.06 and 0.05 mg/mL respectively. The identification of anthocyanins in the extracts of the two sweet potato varieties was performed by HPLC-MS-UV, and HPLC-MS/MS analyses. Four anthocyanin isomers were identified in Tuskegee purple Cap Vert variety. These are : cyanidin 3-sophoroside-5-glucoside ; peonidin 3-sophoroside-5-glucoside ; cyanidin 3-laminaribioside-5-glucoside ; peonidin 3- laminaribioside-5-glucoside.