Rabbit cornea epithelization after the antlerogenic stem cells of Cervus elaphus homogenate

Abstract

AbstractPurpose There are many possibly cause of disturbances of the injured cornea epithelization, like for example corneal limbal stem‐cell deficiency, diabetes. Growing antlers constitute a model organ for examining regeneration processes of tissues because they are the only mammalian appendages capable of regeneration.Methods New Zealand White rabbits were used, one eye of each animal was treated, and the fellow eye was the control. Erosion of the corneal epithelium was performed: after local anesthesia a 3 mm diameter disc soaked in n‐heptanol was placed on the cornea for 30 seconds. Then the eye was washed with a saline. Formulation was made of antlerogenic stem cells of Cervus elaphus cultured under standard conditions. After homogenization of 500000 cells we received 1ml of drops or gel. Rabbits received the homogenate ‐ drops or gel ‐ 3 times a day. The eyes were examined using slit lamp, fluorescein staining and photography of the cornea ‐ test and 10 hours after injury. The surface of the damaged cornea was measured in pixels, analyzing the extent of damage using Adobe Photoshop CS4 ExtendeResults The results were averaged ‐ assessing the reduction in the surface of the wound over time. The experiment was terminated to obtain 100% of the closing of each corneal wound ‐ test and control groups. Corneal healing treated by the investigated preparations followed on the second day and on the 3rd day in the control group. There was observed no magnification the lesion of the injury in case of gel application in the first 10 hours.Conclusion Further experimental and clinical studies of Cervus elaphus stem cells are recommended. They are necessary for evaluation of usefulness to treat corneal injures.