Quingestrone—Determination of Minute Quantities of Decomposition Products by Paper Chromatography

Abstract

A comprehensive paper chromatographic assay procedure has been developed for quingestrone and its decomposition products. The complete assay procedure consists essentially of three steps: (a) separation and identification of the decomposition products, (b) quantitative determination of the decomposition products, and (c) quantitative assay of the parent compound. As little as 1 per cent of decomposition products can be determined with an accuracy of ±5 per cent. Stability data is presented for quingestrone in crystalline form, in solutions, and in the pharmaceutical dosage form. A comprehensive paper chromatographic assay procedure has been developed for quingestrone and its decomposition products. The complete assay procedure consists essentially of three steps: (a) separation and identification of the decomposition products, (b) quantitative determination of the decomposition products, and (c) quantitative assay of the parent compound. As little as 1 per cent of decomposition products can be determined with an accuracy of ±5 per cent. Stability data is presented for quingestrone in crystalline form, in solutions, and in the pharmaceutical dosage form.