An oligonucleotide mapping procedure and its use in the study of tobacco mosaic virus nucleic acid

Abstract

A two-dimensional procedure was developed employing paper electrophoresis and paper chromatography, which permitted the separation and identification of all major products obtained by digestion of tobacco mosaic virus ribonucleic acid (TMV-RNA) with pancreatic ribonuclease. The map so obtained showed nineteen spots accounting for approximately 90 % of the optical density of the material applied. The substances in the major spots were quantitatively estimated by elution and spectrophotometry, and qualitatively identified by application of standard procedures for determining the composition of nucleotides and oligonucleotides. The homogeneities of the isolated compounds were evaluated by elution and subjection of eluates to ion-exchange and paper-chromatographic procedures.