Abstract
Fructose is 30% sweeter than sucrose, the sweetest natural sugar in the world and the main compound of agave syrup. Although fructose can be got from different syrups through a chromatographic system, the methods to evaluate their purification are expensive and too long time. In this work, using a polarimetric method was determinate the fructose-glucose ratio from agave syrup before and after their process by a chromatography method. The polarimetric method was validated using a standard HPLCbased method. With the results of this work was development a rapid and economic technique to determinate the fructose-glucose ration in agave syrup.
Highlights
The fructose is used as a dietary sugar in an increasing variety of products of industrial relevance
Even though the fructose and glucose separation can be achieved by methods as chromatography, cooling crystallization, and reverse osmosis [5]; the evaluation of their separation by high performance liquid chromatography (HPLC), gas chromatography-mass spectrometry (GC-MS), or nuclear magnetic resonance (NMR) [6,7], is expensive and delaying
The Anova variance analysis performed to the results showed in the Table 3, determined that there are no statistically significant differences between the results got through polarimetric and HPLC method, since the F test value does not exceed the critical F value (Table 4)
Summary
The fructose is used as a dietary sugar in an increasing variety of products of industrial relevance. Fructose can be produced by acid or enzymatic hydrolysis of sucrose or through their purification from corn and agave syrups, due this sugar is their main compound. The agave syrup with high quality fructose is clear in color, without aroma agave plant, and had been reported with antioxidant [3] and antibacterial properties [4]. Even though the fructose and glucose separation can be achieved by methods as chromatography, cooling crystallization, and reverse osmosis [5]; the evaluation of their separation by high performance liquid chromatography (HPLC), gas chromatography-mass spectrometry (GC-MS), or nuclear magnetic resonance (NMR) [6,7], is expensive and delaying. The aim of this work was the development of an inexpensive and quick method to measure fructose-glucose ratio from agave syrup before and after a chromatography process
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