Abstract
Coumaphos is a common organophosphorus pesticide used in agricultural products. It is harmful to human health and has a strictly stipulated maximum residue limit (MRL) on fruits and vegetables. Currently existing methods for detection are complex in execution, require expensive tools and are time consuming and labor intensive. The surface plasmon resonance method has been widely used in biomedicine and many other fields. This study discusses a detection method based on surface plasmon resonance in organophosphorus pesticide residues. As an alternative solution, this study proposes a method to detect Coumaphos. The method, which is based on surface plasmon resonance (SPR) and immune reaction, belongs to the suppression method. A group of samples of Coumaphos was detected by this method. The concentrations of Coumaphos in the samples were 0 µg/L, 50 µg/L, 100 µg/L, 300 µg/L, 500 µg/L, 1000 µg/L, 3000 µg/L and 5000 µg/L, respectively. Through detecting a group of samples, the process of kinetic reactions was analyzed and the corresponding standard curve was obtained. The sensibility is less than 25 µg/L, conforming to the standard of the MRL of Coumaphos stipulated by China. This method is label-free, using an unpurified single antibody only and can continuously test at least 80 groups of samples continuously. It has high sensitivity and specificity. The required equipments are simple, environmental friendly and easy to control. So this method is promised for a large number of samples quick detection on spot and for application prospects.
Highlights
Organophosphorus pesticide (OPPs) is a type of phosphate with different substituent groups that cause the inhibition of acetylcholinesterase to produce an insecticidal effect [1,2,3]
The fixation of the bioprobe H11-OVA is shown in Marking 4, and the response value increases drastically
The fixed probe is monitored in real time
Summary
Organophosphorus pesticide (OPPs) is a type of phosphate with different substituent groups that cause the inhibition of acetylcholinesterase to produce an insecticidal effect [1,2,3]. China is one of the world’s leading users of pesticide, nearly 70% of which is organophosphorus. Organophosphorus pesticide often has high levels of neurotoxicity in human and animals through gradual accumulation and regular intake. Organophosphorus pesticide may lead to symptoms such as neurological disorders, tremors, language disorders and even death [4,5,6,7,8,9,10,11,12,13,14,15]. Due to its high levels of toxicity, many organophosphorus pesticides have been banned or highly limited in use by most countries. Efforts are being made to revamp restrictions and regulations of organophosphorus pesticide residues in imported fruits and vegetables across America, Europe and Asia, which makes improved detection methods urgent and necessary
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