Quick and high-throughput quantification of 22 β-agonists residues in animal-derived foods using enzymatic probe sonication

Abstract

A high demand exists in veterinary drug residual analysis for rapid, automatic and high-throughput analytical techniques that produce data simultaneous and faster. Here, we describe a combined automated solid-phase extraction (SPE) and enzymatic probe sonication (EPS), subsequently ultra-performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS) for the quick extraction, automated clean-up as well as simultaneous quantification of 22 β-agonists residues in animal-derived foods. Enhanced by the ultrasonic probe, only 2 min was needed for exhaustively extraction of β-agonists from foods of animal-origin; whereas traditional enzymatic digestion methods require hours or even days, which making it more appropriate for high-throughput biomonitoring. Moreover, the clean-up and pre-concentration procedures were conducted on the automatic SPE, which allowed 36 samples being performed simultaneously within 30 min. The method was successfully applied for analyzing 56 animal-derived food samples, 53.6 % of which contained detectable levels of at least one kind of β-agonists. Interestingly, both the detection rate and residual level of β-agonists in the ruminants (bovine and sheep) were higher as compared with the swine. Concerning the real food samples analyzed, the findings of this study suggest that stricter measures should be adopted to control the illegal usage of β-agonists on the farm animals, particularly for the ruminants.