Quelques applications d'un détecteur viscosimétrique à la chromatographie d'exclusion en phase liquide

Abstract

Some applications of a viscosimetric detector in gel permeation chromatography In this paper a detector which permits direct measurement of the viscosity of the eluate, leading to a more quantitative use of gel permeation chromatography, is described. If the elution volume and the concentration of polymer (determined by refractometry) are known, it is possible, with the use of a gel permeation chromatography universal calibration curve, to determine the molecular weight of each fraction, thus giving rapid, complete and accurate characterization of an unknown polymer. This technique has been applied to: (1) the study of linear polymers and the relation between viscosity and molecular weight; (2) the characterization of branched polymers and the determination of the degree of branching as a function of molecular weight; (3) the study of copolymers of different composition. Here the composition of each fraction was determined with the aid of a third detector, allowing evaluation of polydispersity in weight as well as in composition of the copolymers.