Quantitative Trait Loci Underlying Seed Sugars Content in “MD96-5722” by “Spencer” Recombinant Inbred Line Population of Soybean

Masum Akond,Stella K Kantartzi,My Abdelmajid Kassem,David A Lightfoot,Nacer Bellaloui,Khalid Meksem,Shiming Liu

doi:10.4236/fns.2015.611100

Masum Akond, Stella K Kantartzi + Show 5 more

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https://doi.org/10.4236/fns.2015.611100

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Abstract

Sucrose, raffinose, and stachyose are important soluble sugars in soybean [Glycine max (L.) Merr.] seeds. Seed sucrose is a desirable trait for taste and flavor. Raffinose and stachyose are undesirable in diets of monogastric animals, acting as anti-nutritional factors that cause flatulence and abdominal discomfort. Therefore, reducing raffinose and stachyose biosynthesis is considered as a key quality trait goal in soy food and feed industries. The objective of this study was to identify genomic regions containing quantitative trait loci (QTL) controlling sucrose, raffinose, and stachyose in a set of 92 F5:7 recombinant inbred lines (RILs) derived from a cross between the lines “MD96-5722” and “Spencer” by using 5376 Single Nucleotide Polymorphism (SNP) markers from the Illumina Infinium SoySNP6K BeadChip array. Fourteen significant QTL were identified and mapped on eight different linkage groups (LGs) and chromosomes (Chr). Three QTL for seed sucrose content were identified on LGs N (Chr3), K (Chr9), and E (Chr15). Seven QTL were identified for raffinose content on LGs D1a (Chr1), N (Chr3), C2 (Chr6), K (Chr9), B2 (Chr14), and J (Chr16). Four QTL for stachyose content were identified on LG D1a (Chr1), C2 (Chr6), H (Chr12), and B2 (Chr14). Selection for beneficial alleles of these QTLs could facilitate breeding strategies to develop soybean lines with higher concentrations of sucrose and lower levels of raffinose and stachyose.

Highlights

Soybean [Glycine max (L.) Merr.] seeds are used in many prepackaged traditional foods such as soy milk, tofu, tofu skin, soy sauce, bean paste, natto, and tempeh
Since limited research was done in this area of quantitative trait loci (QTL) mapping for seed sugars, the objective of this study was to identify more QTLs related to seed sucrose, raffinose, and stachyose QTLs based on natural variation of seed sugars
Our research shows that out of 20 linkage groups (LGs)/Chr of soybean we identify 14 QTL on 8 different LG/Chr which underlie the three main sugars in the MD96-5722 by Spencer recombinant inbred lines (RILs) population of soybean

Summary

Introduction

Soybean [Glycine max (L.) Merr.] seeds are used in many prepackaged traditional foods such as soy milk, tofu, tofu skin, soy sauce, bean paste, natto, and tempeh. These types of foods are produced from special soybean lines that are ideally low in oil but high in sucrose and proteins. The di-, tri-, and tetra-saccharides: sucrose, raffinose, and stachyose are the most abundant soluble sugars in soybean seeds [1], accounting for 5%, 1.5%, and 3% of the total carbohydrates portions (35%) [2]-[4]. Seed sugar content is an important trait in food-grade soybeans and up to 10% sucrose and less than 2.5% total raffinose and stachyose contents are considered advantageous [4]

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