Abstract
Limited information is available on the genetic analysis of amino acid composition in soybean seeds. Previously, quantitative trait loci (QTLs) for seed isoflavones, protein, oil, and fatty acids were identified in the MD96-5722 by Spencer and other RIL populations. There were wide variations for these seed constituents among the RIL populations. Therefore, the objective of this study was to identify QTLs controlling different amino acids content in soybean seeds. To achieve this objective, ninety-two F5:7 recombinant inbred lines (RIL), developed from a cross of MD96- 5722 and Spencer, using a total 5376 Single Nucleotide Polymorphism (SNPs) markers, were used. The RILs were genotyped by using 537 polymorphic, reliably segregating SNP markers, developed from the Illumina Infinium SoySNP6K BeadChip array. A total of 13 QTLs were identified with three QTLs for threonine on the linkage group (LG) A1, C2, and B2. Two QTLs were identified for each of the amino acids proline on LG D1a and B2, serine on LG A1 and C2, tryptophan on LG K and G, and cysteine on LG A1 and K. One QTL was identified for arginine on LG N and histidine on LG J. The new QTLs findings for seed amino acid will facilitate the development of soybean cultivars with higher protein and amino acid quality to help meet the industry and consumer needs. * Corresponding author.
Highlights
Soybean [Glycine max (L.) Merr.] is a legume crop, and its seeds are among few plant-derived food sources that contain useful concentrations of all essential amino acids [1]
Cysteine is a sulfur-containing amino acid that is generally deficient in soybean seed cultivars, and higher CYS is a desirable trait, and variation among our recombinant inbred lines (RIL) may provide an opportunity for further research to select for higher sulfur-containing Amino acids (AA) (CYS)
A linoleic acid QTL was detected by others elsewhere [7] on linkage group (LG) N (Chr3) at 4.80 cM position was very close to the QTL for ARG at 4.00 cM; the QTL we identified for SER on LG C2 (Chr6) has been identified for raffinose at the same position (7.20 cM)and the QTL identified in LG K (Chr 9) for SER and TRP (0.50 cM) were very close to QTL identified in a previous study for the seed content of the isoflavone glycitein
Summary
Soybean [Glycine max (L.) Merr.] is a legume crop, and its seeds are among few plant-derived food sources that contain useful concentrations of all essential amino acids [1]. Genomic regions associated with amino acid composition in soybean were identified using 101 F6-derived recombinant inbred lines developed from a cross of N87-984-16 x TN93-99 [11] They found a significant (p < 0.01) difference among the RIL for amino acid composition and a total of 94 polymorphic simple sequence repeats (SSRs) molecular markers. They analyzed essential and non-essential amino acid composition in soybean seed proteins by using 282 F5:9 recombinant inbred lines developed from a cross of Essex × Williams 82 [11] They used the Universal Soy Linkage Panel (USLP) 1.0 of 1536 single nucleotide polymorphism (SNP) DNA markers to genotype the 282 RILs and identify 480 useful genetic markers [11]. They concluded that detecting QTLs for seed amino acids in soybean would provide useful information to select genotypes with improved seed amino acid qualities for human nutrition and livestock production [11]
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