Abstract
The ability of flow cytometry to detect intracellular accumulation of poly(β-hydroxybutyrate) (PHB) in individual cells of Rhizobium meliloti was investigated using light scatter parameters. PHB accumulation during stationary phase was shown to form inclusion bodies inside cells which were detected by forward angle light scatter (FALS) parameter. A linear relationship was found between FALS intensity and percentage of PHB (w/w) of cells, demonstrating that flow cytometry allowed a quantitative analysis of intracellular PHB accumulation. In addition, results showed the ability of flow cytometry to quantify heterogeneity of rhizobia population in fermentation according to PHB production and accumulation.
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