Abstract
Several single histone modifications are associated with both gene activation and silencing, but what type of effect distinct combinations of simultaneously occurring histone modifications (Histone Codes or patterns) have upon cellular events is poorly understood. Additionally, most recent research describes histone modifications as static events. The main reason for this lack of knowledge is that techniques for quantitative characterization or even qualitative identification of combinatorial Histone Codes in dynamic fashion by any method do not readily exist. We have specifically addressed this deficiency by developing novel mass spectrometry based methods for rapid comparison of dynamically changing histone modifications from multiple cellular states including quantitative tracking of hundreds of combinatorial Histone Codes in a single experiment. These studies in combination with biological experiments will help provide a systems‐wide outlook that will lay down the basic scientific foundation to advance several areas of chromatin biology.
Published Version
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