Abstract
Mammary glands provide an ideal system for post-genomic study for diary production as there is a dramatic proliferation and expansion of the organelles involved in milk production during transition from dry to maximal milk secretion. The current study reports the use of subcellular fractionation and label-free “shotgun” proteomics to examine differential expression of proteins in mammary glands from three experimental groups of cows. We have identified 441, 393, 295 and 338 proteins in subcellular fractions of cytosol, membrane, nucleus and cytoskeleton respectively, of which 159, 84, 144 and 114 are unique to each fraction. Spectral counting and DeCyder MS (GE Healthcare, Life Sciences) analysis showed that 16%, 11.5%, 18.5% and 21.8% of proteins identified in cytosol, membrane, nucleus and cytoskeleton fractions varied more than 1.5-fold in abundance among the experimental groups. Of these differentially expressed proteins, we are particularly interested in the proteins that affect milk lipid production such as stearoyl-coenzyme A desaturase, acetyl-CoA acetyltransferase, fatty acid binding protein 5 and apolipoprotein A1 etc, which are supposed to be related to the phenotypes of the three biological groups of cows. These data provide the background for using proteomics to discover genes with metabolic effects. doi:10.4172/jpb.s1000213
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