Quantitative phase imaging with single shot digital holography

Abstract

We demonstrate quantitative phase imaging using single shot digital holography for a calibrated spiral phase object. A single frame of near on-axis digital hologram of a spiral phase plate is recorded and the complex object field in the hologram plane is retrieved using a constrained optimization approach. Experimental results show the feasibility of a quantitative phase imaging technique which has superior performance to conventional Fourier filtering methods. Single shot capability suggests that this method is suitable for holographic imaging of dynamic objects such as live biological cells.