Quantitative PCR validation of hormones and growth and immune factors from gene array of rat cecal cells fed resistant starch and control diets

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Previously we reported the gene array results on cecal cells from rats fed three diets: resistant starch (RS, 3.2 kcal/g), standard control (CC, 3.6 kcal/g), and energy balanced control (EC, 3.2 kcal/g). We now can report the validation assay performed by quantitative PCR (n=8) for 15 hormones, and growth and immune factors with the greatest fold changes and levels of significance from the gene array. The 18S ribosomal RNA gene was used as the endogenous control. Three t‐tests, RS‐EC, RS‐CC, and CC‐EC, were performed with results for RS‐EC presented. Fourteen of 15 factors found different by the gene array were validated. Large increases (>2) in fold change and highly significant (p<0.0001) differences for RS were: 1) hormones ‐ adrenomedullin, glucagon, neurotensin and peptide YY; 2) growth factors ‐ amphiregulin, bone morphogenetic protein 2, epiregulin, and vascular endothelial growth factor alpha; and 3) immune factor ‐ interleukin 1 alpha. Results demonstrate dietary RS is associated with increased gene expression of hormones and factors that may positively impact the health of the gut and body of the animals. Funding sources were National Starch and LSU AgCenter.