Abstract
Proteases break peptide bonds. It is often necessary to measure and/or compare the activity of alkaline proteases using different procedures in the lab. Many studies with keen interest on alkaline proteases mostly use quantitative and/or qualitative assay methods to assay the enzyme activity of proteases. There is need to select a suitable assay method from the reported ones which will be ideal for any proposed study. There could be challenges when choosing the right assay method from the existing ones, thereby prompting the need for a review of the various methods for the quantitative assay of alkaline protease, the quantitative methods and protocols used from 1938 until now and their industrial applications were chronologically reviewed.
Highlights
Microorganisms are known to play a vital role in technology for the production of intracellular and extracellular enzymes on industrial scale [1, 2]
1.1 Proteases (EC 3:4, 11-19, 20-24, 99) constitutes a large and complex group of enzymes [8]. This is the group of enzyme which carries out proteolysis [9]
The present study aimed to review the most applicable quantitative assays methods of alkaline protease enzymes
Summary
Microorganisms are known to play a vital role in technology for the production of intracellular and extracellular enzymes on industrial scale [1, 2]. 1.1 Proteases (EC 3:4, 11-19, 20-24, 99) constitutes a large and complex group of enzymes [8]. This is the group of enzyme which carries out proteolysis (the breakdown of proteins through the hydrolysis of the peptide bond that exists between two amino acids of polypeptide chains) [9]. Proteases are ubiquitous and widely distributed, found in a wide diversity of sources such as plants, animals, and microorganisms. Alkaline proteases are important industrial enzymes which can be produced by many bacterial genera such as Aeromonas, Alcaligenes, Arthrobacter, Bacillus, Halomonas, Pseudomonas, and Serratia [12]
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