Abstract
The Ehrlich method for the determination of indole-like compounds was expanded by the appropriate control of acidity and oxidation to differentiate between anthranilic acid, indole, and tryptophan. This method afforded a convenient quantitative determination of these three compounds in the same aliquot. This determination depends upon the reactions of anthranilic acid and indole with p-dimethylaminobenzaldehyde (PDAB) in 0.5 N acid to form colored products. The formation of a colored derivative of tryptophan with PDAB requires the presence of much higher acid concentration (ca. 11 N) and an oxidizing agent. The color formation does not appear to be affected by the presence of constituents of a synthetic Lactobacillus medium.
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