Abstract
An immunoelectrofocusing technique is described applying the principle of antigen-antibody crossed electrophoresis. The method can be used for characterization as well as quantitation of proteins with regard to their heterogeneity. Using this technique, the immunoelectrofocusing behaviour of purified preparations of human serum albumin and human serum Zn-α 2- glycoprotein was studied. Two components of the human serum Zn-α 2- glycoprotein differing in their isoelectric points but antigenically identical were observed; the ratio of the variants in the purified human serum Zn-α 2- glycoprotein preparation was found to be approximately 2:1. The quantitative immunoelectrofocusing method described might be useful for the investigation of the homogeneity of purified proteins and for the study of the heterogeneity and quantitation of proteins which may appear homogeneous by other criteria.
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