Abstract
Abstract A method was developed for determination of salicylic acid in anti-acne pharmaceutical preparations involving separation on a preadsorbent high performance thin layer chromatography (HPTLC) silica gel plate with fluorescent phosphor, detection by fluorescence quenching, and quantification by densitometric scanning. Salicylic acid was directly detected and quantified on the plate at levels as low as 1 μg, and no interference was encountered from other ingredients in the medication formulations. The method was applied to the analysis of a commercial gel preparation with a label value of 2.0% salicylic acid, and an average value of 99.2% of theoretical was obtained (n=16). Accuracy was validated by analysis of a 10% benzoyl peroxide anti-acne gel spiked with 2% salicylic acid, and recovery averaged 100.0% (n=10). The coefficients of variation for the analyses were 3.9 and 5.6%, respectively. Advantages of the new HPTLC method are discussed.
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