Quantitative Estimation of Secondary Metabolites of Shadanga Paniya Classical Ayurvedic Formulation and Constituent Herbs

The hawthorn Crataegus mexicana is a traditional Mexican fruit with properties that make this fruit useful for the treatment of many ailments, including diseases of the respiratory and urinary tract. This paper reports the antioxidant capacity of the n-hexane, dichloromethane, ethyl acetate, acetone, ethanol and methanol extracts of C. mexicana. Samples were evaluated for total phenolic and carotenoid contents, 2,2-diphenyl-1-picryl-hydrazyl (DPPH) radical scavenging, the inhibition of the formation of thiobarbituric acid reactive species (TBARS) and the neutralization of the cation-radical 2,2´-azino-bis(3ethyl benzothiazoline-6-sulfonic acid) diammonium salt (ABTS). The total phenolic content was 2.65 ± 0.23 mg of gallic acid equivalents per gram, and the carotenoid content was 26.4 ± 0.02 µg/g in dry hawthorn skin. The most active extract in scavenging DPPH radicals and inhibiting TBARS formation was the acetone extract, with activities of 21.9 ± 0.15 and 13.27 ± 0.70%, respectively, at 10 mg/L. The extracts were compared for activity against ascorbic acid, caffeic acid, α- tocopherol and quercetin. The acetone extract was the most active, with an IC50 value of 15.2 mg/L in DPPH and 17.7 mg/L in TBARS. A high correlation was observed between the results for TBARS and DPPH. These results demonstrate the potential nutritional and antioxidant value of this Mexican fruit.

Background: Legumes and cereals microgreens are consumed both in healthy nutrition and as a natural food against many degenerative diseases due to their rich bioactive content. The research was carried out to determine the biochemical contents of 5 legumes and 7 cereals. Methods: The research was conducted in Van Yuzuncu Yıl University Field Crops Department Laboratory in 2020, in a controlled growing environment, according to the completely randomized experimental design. Total antioxidant activity, total phenolic, total flavonoid and total ascorbic acid contents and total Chlorophyll, Chlorophyll a, Chlorophyll b and Carotenoid contents and correlation between them were determined. Result: The differences between the means of all traits were found to be significant. The biochemical contents of cereals were 1.6-7 times higher than legumes. There was no big difference between the pigment values. In legumes, Bilensoy alfalfa had the highest total antioxidat activity and total flavonoid content, Uzbek lentil total phenolic content and Goynuk bean had the highest Total ascorbic acid. In cereals, Kirklar oat had the highest total antioxidat activity, total phenolic, total flavonoid and total ascorbic acid contents, while Larende barley had the lowest values (except total ascorbic acid content). While total Chlorophyll and Chlorophyll a amounts were highest in Kirklar barley and lowest in Uzbek lentils, the opposite situation occurred in Chlorophyll b and Carotenoid amounts. There was no significant correlation between biochemical parameters in legumes. There was a positive and significant correlation between total phenolic content with total antioxidant activity and between total ascorbic acid with total fenolic content in cereals. There were positive and significant correlations between all pigment parameters in both legumes and cereals.

The current study was aimed to determine the total phenolic content and antioxidant activity, rutin, quercetin and phenolic acids content in buckwheat, husked and common oats, winter spelt, winter and spring wheat grain with and without husks or glume, only husks or glume as well as to estimate phenolic compounds ratio and to compare their distribution between cereal grain and husks or glume.In this way to ascertain the losses of phenolic compounds in grain which will be used for food production.Total phenolic content and antioxidant activity were determined using the spectrophotometric method and individual phenolic compounds were estimated by high performance liquid chromatography.The significantly highest total phenolic content (10.2 ± 1.6 mg g -1 dry weight (d.w.) in grain with husks and 14.1 ± 2.0 mg g -1 d.w. in husks), antioxidant activity (2-4 times), rutin concentration (from 146.5 to 406.3 µg g -1 d.w.) and with a few exceptions hydroxybenzoic acids concentrations of all group of cereal samples (grain with and without husks or glume and only husks or glume) were established for buckwheat.Oats grain without husks or only husks were distinguished by the highest quercetin content, which was higher in husked oats samples (31-89 µg g -1 d.w.).The highest total phenolic acids content was in common oats husks (882.4 µg g -1 d.w.).Ferulic and p-coumaric acid significantly (P < 0.01) predominated in all oats and wheat samples and accounted for about 70-90% of the total phenolic acids content.Syringic acid is characteristic of oats, winter spelt and winter wheat grain without glume and only glume.In all group of cereal samples sinapic acid predominated only in cereal grain with and without husks or glume.Significantly (P < 0.05) higher concentrations of vanillic, p-hydroxybenzoic, 3,4-dihydroxybenzoic, p-coumaric, ferulic acids, total phenolic content, antioxidant activity were quantified in cereal husks or glume compared with those in grain with and without husks or glume.

Phenolic and flavonoid contents of mandarin (Citrus reticulata Blanco) fruit tissues and their antioxidant capacity as evaluated by DPPH and ABTS methods

Phenolic and flavonoid contents in plants play a great role in scavenging free radicals in the body and act as antioxidants; thus making their determination very vital. Total phenolic and flavonoid contents of the methanol extract of Voacanga africana root bark and its fractions (n-hexane, ethyl acetate and n-butanol) were carried out in this study. The total phenolic content was determined by using Folin-Ciocalteau assay while the the total flavonoid content was determined by the aluminium chloride colorimetric assay method. The result obtained showed that n-hexane fraction of the plant exhibited the highest (116.607±95.13 mgGAE/g) total phenolic content (TPC) at all concentrations followed by ethyl acetate fraction of the plants. The highest total flavonoid content across concentrations (300, 250, 200, 100, 50 and 25 μg/ml) was exhibited by n-hexane fraction (467.143±44.22 mg QE/g). From the results, it was concluded that n-hexane fraction of V. Africana root bark exhibited the highest total phenolic and total flavonoid contents at all concentrations (ppm), followed by ethyl acetate. It could be deduced that V. africana root bark possesses phenolic and flavonoid contents depending on the type of extract or fraction; thus justifying its folkloric use in the treatment of diseases.Keywords: Phytochemicals, Phenols, Flavonoids, Voacanga africana, Root bark, Fraction

In order to further develop and utilize the rutin from Sophora japonica, the contents of total phenolic acids and flavonoids in rutin extracts from different parts of Sophora flavescens were determined, and their antioxidant activities were compared, and the basis of antioxidative activity of Sophora flavescens nanometers was discussed. The experiment found that the total flavonoid content of each extract of Huai Mi was in the range of 5.70–61.55 mg/g, the total phenolic acid content was in the range of 4.07–19.10 mg/g, and the total flavonoid and phenolic acid content of the ethyl acetate extract was significantly higher than other extracts. Its reducing power, light free radicals, DPPH, ABTS+, and scavenging rate are also significantly higher than n-butanol, petroleum ether, and aqueous extracts. ∗The cleaning ability IC50 values of DPPH· are (0.321 ± 0.0026), (0.213 ± 0.0010), and (0.169 ± 0.0014) mg/mL. The effective components of Sophora japonica are initially separated after the solvent extraction of each phase. The relative content and nanoantioxidant activity of total flavonoids and phenolic acids in the ethyl acetate phase extraction part were significantly stronger than those in the total extract and other extraction parts, and the antioxidant capacity was effectively related to the dose. The flavonoids and phenolic acids in Sophora japonica are closely related to its antioxidant capacity.

Bunium persicum Boiss. seeds have been used for medicinal and nutritional properties such as antioxidant, antihelmetic and antimicrobial activity. The aim of this study was to to tested protective role of cumin extract against abiotic stress by microRNA markers. Secondary also was to evaluate antioxidant activity as well as total polyphenol, flavonoid and phenolic acid content of cumin extract. We observed that cumin DNA itself has not been damaged by sonication teratment. This protective impact indicates that cumin antioxidant properties can efficiently quench free radicals induced by sonication. On the other side, ultrasound-mediated formation of reactive oxygen species did induce the DNA polymorphism of lettuce samples which was detected by miRNAs-based markers. The range of sonication impact was time-dependent. Markers based of miRNA-DNA sequences has proven to be an effective tool. We have confirmed statistically significant differences (p ≤0.01) in miRNAs markers ability to detect the polymorphism due to sonication treatment. The antioxidant activity was determined by a method using DPPH radical and phosphomolybdenum method, total polyphenol content with Folin - Ciocalteu reagent, total flavonoid with aluminium-chloride mehod and total phenolic acid with Arnova reagent. Results showed that cumin is rich for biologically active substances and can be used more in different kind of industry as a cheap source of these substances. Antioxidant activity with DPPH method was 1.18 mg TEAC.g-1 (TEAC - Trolox equivalent antioxidant capacity per g of sample) and by phosphomolybdenum method 45.23 mg TEAC.g-1. Total polyphenol content achieved value 4.22 mg GAE.g-1 (GAE - gallic acid equivalent per g of sample), total flavonoid content value 10.91 mg QE.g-1 (QE - quercetin equivalent per g of sample) and total phenolic acid content value 5.07 mg CAE.g-1 (CAE - caffeic acid equivalent per g of sample).

ABSTRACT. Pumpkins are increasingly regarded as functional foods since they contain valuable nutrients and bioactive substances that have positive health effects. The number of bioactive components depends on the origins and parts of the pumpkin, which define its antioxidant capacity. This study evaluated the total phenolic, total flavonoid, and ascorbic acid content and antioxidant activities of three parts of pumpkin from four different sample origins and investigated the correlation between them. The content of bioactive components in pumpkin from Dukem, Ethiopia, was highest in the flesh, peel, and seeds compared to the other sample areas. The Pearson correlation result indicated a strong relationship between the total phenolic, flavonoid, and ascorbic acid contents of pumpkin and their respective antioxidant activities. This study found that the total phenolic, total flavonoid, and ascorbic acid contents and antioxidant activity varied across the pumpkin sample sources and pumpkin parts.&#x0D; &#x0D; KEY WORDS: Pumpkin, Flavonoid, Phenolic, Ascorbic acid, Antioxidant activity, Ethiopia&#x0D; &#x0D; Bull. Chem. Soc. Ethiop. 2023, 37(5), 1093-1108. &#x0D; DOI: https://dx.doi.org/10.4314/bcse.v37i5.3

Senna didymobotrya has been used in Kenya by the Kipsigis community to control malaria as well as diarrhoea. The Pokot prepare charcoal from the stem for milk preservation. Research has not been done to investigate the effect of different extraction solvents on yield, total phenolic and flavonoid content of Senna didymobotrya plant roots. The aim of this study was to compare root extract yield of diethyl-ether, methanol, and aqueous solvents; phytochemical screening; and total phenolic and flavonoid content of Senna didymobotrya plant roots. Extraction was done by the Soxhlet method. Phytochemical screening was done using Harborne's (1973) method with a slight modification. Total flavonoid content was determined by aluminium chloride colourimetric assay at 420 nm. Total phenolic content was determined by Folin–Ciocalteu at 760 nm using UV-Vis spectrophotometry. Extraction yield of diethyl ether, methanol, and distilled water were 3.72 g (7.44%), 4.97 g (9.94%), and 9.09 g (18.18%), respectively, showing a significant difference (p &lt; 0.05) in the yields obtained using the different solvents. Phytochemical screening was positive for phenols, tannins, saponins, gladiac glycosides, anthraquinones, alkaloids, and flavonoids. Total flavonoid content was found to be 48.3 ± 1.5 (QEmg/g) and total phenol content was calculated as 34.5 ± 0.1 (GAEmg/g). Distilled water can be utilized as the best extraction solvent. Senna has a high amount of flavonoid and phenolic content. The limitation of this research is that it only tested root extracts and not leaves, flowers, or seeds. More studies need to be done to isolate the different compounds identified.

Six cultivars of kiwifruits grown in Korea, including Actinidia eriantha ‘Bidan’, A. arguta ‘Chiak’, A. arguta ‘Darae No. 2’, A. chinensis ‘Haegeum’, A. chinensis ‘Haehyang’, and A. arguta × A. deliciosa ‘Mansoo’, were harvested at various maturity stages to test whether kiwifruit maturity has an influence on antioxidant capacity or total phenolic and flavonoid contents. Kiwifruit extracts were isolated using absolute methanol and then 80% (v·v-1) aqueous methanol during homogenization. ‘Bidan’, collected at the second harvest stage, contained the greatest amount of total phenolics (775.3 mg gallic acid equivalents·100 g-1 fresh weight) and had the highest antioxidant capacity [816.5, 633.2, and 2,662.7 mg vitamin C equivalents·100 g-1 fresh weight for 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) scavenging, 1,1-diphenyl-2-picrylhydrazyl scavenging, and oxygen radical absorbance capacity assays, respectively] among cultivars tested, while ‘Haehyang’, collected at the first harvest, contained the greatest amount of total flavonoids (13.1 mg catechin equivalents·100 g-1 fresh weight). Kiwifruit cultivar and genotype influenced antioxidant capacity, as well as total phenolic and flavonoid contents. No trend, however, was observed in total phenolic and flavonoid contents, and in the antioxidant capacity with respect to maturity stage. Antioxidant capacity had a higher linear correlation coefficient with total phenolic contents than with total flavonoid contents. The results above suggest that kiwifruits at various maturity stages are a valuable source of phenolics and antioxidants for industrial application and consumer health benefit.

Aim: The current study was undertaken to determine the total phenolic and flavonoid contents and to assess the antioxidant activity of two different extracts (flour and oil) of two Algerian Quercus species, Quercus ilex L. and Quercus suber L. Methods and Material: The oil extraction of the two species was achieved using the Soxhlet method. The obtained extracts were estimated for the chemical and physical constants (acidity, peroxide value, iodine value, and ultraviolet absorption indices). Total phenolic content was measured by spectrophotometry according to the Folin-Ciocalteu procedure and calculated as gallic acid equivalents (GAE). The studied extracts were submitted to an estimation of their flavonoid contents too, using aluminum chloride methods. Antioxidant ability was assessed by means of two distinct methods (DPPH• and ABTS•+). Results: The obtained results revealed that antioxidant properties, total phenolic, and total flavonoid contents differed significantly among selected species and extracts. The flour samples possessed the highest level of total phenolic contents (1101–1464 mg GAE/kg dry weight) and exhibited the highest antioxidant capacities with average values of 52.62–40.78 μmol TE g−1 dry weight and 36.19–44.50 μmol TE g−1 dry weight for DPPH and ABTS assays, respectively. Acorn oil extracts showed also remarkable antioxidant activity, up to 2.69 and 3.23 μmol TE g−1 oil (DPPH and ABTS test, respectively), even though the total phenolic contents were low (195.64–322.06 mg GAE /kg of oil). Total phenolic amounts were positively correlated with the antioxidant properties of Quercus flour and oil. Conclusions: Our study provides basic information on the presence of bioactive compounds and antioxidant capacity in acorn fruits, in order to consider their extracts as functional food ingredients and potential source of natural antioxidants. Keywords: Quercus ilex L., Quercus suber L., Total phenolic, Total flavonoid, Antioxidant activity.

Phenolic acids are one of several classes of naturally occurring antioxidant compounds found in sweetpotato. Simplified but reliable methodologies were developed to quantitate total and individual phenolic acids in sweetpotato roots. Total phenolic acid content was measured using both Folin-Denis and Folin-Ciocalteu reagents. The Folin-Ciocalteu reagent gave an overestimation of total phenolic acids due to the absorbance of interfering compounds (i.e., reducing sugars and ascorbic acid). The average total phenolic acid content in `Beauregard' sweetpotatoes was 60.9 mg/100 g fresh weight. Individual phenolic acids were separated with two reversed-phase C18 columns of different dimensions and particle size. The columns tested were a 7 × 53 mm, 3 μm, Alltima Rocket (Alltech Assoc.) and a 3.9 × 150mm, 4 μm, Nova-Pak (Waters Corp.). Different mobile phases were also evaluated. The Alltima C18 column using a mobile phase of 1% (v/v) formic acid aqueous solution: acetonitrile: 2-propanol, pH 2.5 (70:22:8) provided the best separation of individual phenolic acids. Total analysis time was less than 5 minutes. Chlorogenic acid was the major phenolic acid found in sweetpotato root tissue (15.8 mg/100 g fresh weight). In a comparison of different tissue preparation states (fresh, frozen, freeze-dried), fresh tissue gave the highest concentration of total and individual phenolic acids. Among the 3 extraction solvents tested (80% methanol, 80% ethanol, and 80% acetone), 80% methanol and 80% ethanol gave higher, but similar, phenolic acid extraction efficiency.

Oxidative stress is one of the significant precursors of various metabolic diseases such as diabetes, Parkinson’s disease, cardiovascular diseases, cancer, etc. Various scientific reports have indicated that secondary plant metabolites play an important role in preventing oxidative stress and its harmful effects. In this respect, this study was planned to investigate the phenolic profile and antioxidant and antidiabetic potentials of the aqueous extracts from Turkish Cistus species by employing in vitro methods. In vitro digestion simulation procedure was applied to all extracts to estimate the bioavailability of their phenolic contents. Total phenolic, flavonoid, phenolic acid and proanthocyanidin contents were determined for all phases of digestion. In addition, changes in the quantity of the assigned marker flavonoids (tiliroside, hyperoside and quercitrin) were monitored by High-Performance Thin Layer Chromatography (HPTLC) analysis. The antioxidant activity potentials of the extracts were studied by various methods to reveal their detailed activity profiles. On the other hand, in vitro α-amylase and α-glucosidase enzymes and advanced-glycation end product (AGE) inhibitory activities of the extracts were determined to evaluate the antidiabetic potentials of extracts. The results showed that aqueous extracts obtained from the aerial parts of Turkish Cistus species have rich phenolic contents and potential antioxidant and antidiabetic activities; however, their bioactivity profiles and marker flavonoid concentrations might significantly be affected by human digestion. The results exhibited that total phenolic contents, antioxidant activities and diabetes-related enzyme inhibitions of the bioavailable samples were lower than non-digested samples in all extracts.

Whole wheat consumption is associated with reduced risk of chronic diseases. This study determined total phenolic content (TPC), total flavonoid content (TFC), free radical scavenging activities, metal chelating activity, and phenolic acid composition of 12 hard red winter wheat varieties. TPC, TFC, antioxidant activity, and metal chelating activity varied significantly among different varieties. Trans-ferulic acid, syringic acid, vanillic acid, p-coumaric acid, sinapic acid, and 4-hydroxybenzoic acid were detected and quantified in free, conjugated, and bound fractions. Overall, LCS Mint and WB Grainfield varieties have higher phenolic and flavonoid content. Everest, SY Monument and T158 varieties have stronger antioxidant activity. Positive correlations were obtained between total phenolic acid content and antioxidant activity. Our result suggested that phenolic acid composition and antioxidant activity of hard wheat are highly dependent on their genotypes. Practical applications The health benefits of whole grain products are in part attributed to their unique antioxidant activities. Phenolic compounds, especially phenolic acids, are believed to be one of the major contributors to the antioxidant activity of cereal grains. The breeders and food industry can be benefited by a comprehensive understanding of phenolic compositions and antioxidant activities of different wheat varieties. Identifying wheat varieties with significant levels of phenolic content and strong antioxidant activity has the potential not only to promote the value-added cultivation and use of wheat rich in these factors, but also to provide health benefit to consumers, thereby enhancing wheat profitability, agricultural economy, and public health.

Objective: Sterculia quadrifida R. Br. of Sterculiaceae family is locally known as “Faloak” in East Nusa Tenggara Province, Indonesia. S. quadrifida is used in folk medicine to treat hepatitis, rheumatism, and to recover stamina. The aim of this study was to determine the total flavonoids, total phenolic content (TPC), and antioxidant activity of extracts from different plant parts of S. quadrifida.&#x0D; Methods: The sampled parts of S. quadrifida were non-stripped stem bark, new regrown stem bark, old regrown stem bark, root bark, branch bark, and leaves. Stem bark was classified into three categories, namely, bark that has never been peeled (non-stripped stem bark), old regrown stem bark (estimated to be &gt;6 months after debarking), and new regrown stem bark (estimated to be &lt;6 months after debarking). Total flavonoid content (TFC) was determined by colorimetric aluminum chloride method and TPC was measured using Folin–Ciocalteu’s reagent. Antioxidant activity was determined with 2,2-diphenyl-1-picrylhydrazyl (DPPH).&#x0D; Results: The concentrations of flavonoids in S. quadrifida extracts from different plant parts varied from 0.58±0.13 to 1.25±0.10 mg QE/g. The TPC in the extracts of different plant parts ranged between 8.61±0.09 and 10.43±0.08 mg GAE/g. Branch bark has the highest total flavonoid and phenolic content. The extract of new regrown stem bark exhibited potent antioxidant activity with inhibitory concentration (IC50) values of 2.51±0.03 μg/ml.&#x0D; Conclusion: This study demonstrated for the first time that extracts from different plant parts of S. quadrifida exhibited strong antioxidant activity. However, the total phenolic and flavonoid contents in S. quadrifida only indicated a weak correlation with its antioxidant activity.